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Literature summary extracted from

  • Prabhakar, V.; Capila, I.; Bosques, C.J.; Pojasek, K.; Sasisekharan, R.
    Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant expression and active site identification (2005), Biochem. J., 386, 103-112.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.21 NaCl optimal concentration 62.5 mM for substrate chondroitin 6-sulfate, inhibitory above 125 mM, optimal concentration 125 mM for substrate dermatan sulfate, inhibitory above 250 mM Proteus vulgaris
4.2.2.21 Sodium acetate required Proteus vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.21 E653A no enzymic activity Proteus vulgaris
4.2.2.21 H501A no enzymic activity Proteus vulgaris
4.2.2.21 H561A increase in Km-values Proteus vulgaris
4.2.2.21 H712A increase in Km-values Proteus vulgaris
4.2.2.21 R500A increase in Km-values, large decrease in kcat values Proteus vulgaris
4.2.2.21 R560A no enzymic activity Proteus vulgaris
4.2.2.21 Y508A no enzymic activity Proteus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.21 NaCl optimal concentration 62.5 mM for substrate chondroitin 6-sulfate, 50% inhibition at 125 mM, optimal concentration 125 mM for substrate dermatan sulfate, 50% inhibition at 250 mM Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.21 0.0012
-
 chondroitin 6-sulfate wild-type, pH 8.0 Proteus vulgaris
4.2.2.21 0.0015
-
 chondroitin 4-sulfate wild-type, pH 8.0 Proteus vulgaris
4.2.2.21 0.0025
-
 dermatan sulfate wild-type, pH 8.0 Proteus vulgaris
4.2.2.21 0.0051
-
 dermatan sulfate mutant H712A, pH 8.0 Proteus vulgaris
4.2.2.21 0.0069
-
 dermatan sulfate mutant H561A, pH 8.0 Proteus vulgaris
4.2.2.21 0.0086
-
 chondroitin 6-sulfate mutant H712A, pH 8.0 Proteus vulgaris
4.2.2.21 0.015
-
 chondroitin 6-sulfate mutant H561A, pH 8.0 Proteus vulgaris
4.2.2.21 0.02
-
 chondroitin 6-sulfate mutant R500A, pH 8.0 Proteus vulgaris
4.2.2.21 0.036
-
 dermatan sulfate mutant R500A, pH 8.0 Proteus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.21 110000
-
 x * 110000, SDS-PAGE, x * 112614, calculated Proteus vulgaris
4.2.2.21 112614
-
 x * 110000, SDS-PAGE, x * 112614, calculated Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.21 Proteus vulgaris
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.21 14.8
-
 substrate hyaluronan, pH 8.0 Proteus vulgaris
4.2.2.21 34.8
-
 substrate chondroitin sulfate E, pH 8.0 Proteus vulgaris
4.2.2.21 54.2
-
 substrate chondroitin sulfate D, pH 8.0 Proteus vulgaris
4.2.2.21 69.8
-
 substrate chondroitin, pH 8.0 Proteus vulgaris
4.2.2.21 122.3
-
 substrate dermatan sulfate, pH 8.0 Proteus vulgaris
4.2.2.21 174.6
-
 substrate chondroitin 6-sulfate, pH 8.0 Proteus vulgaris
4.2.2.21 290.6
-
 substrate chondroitin 4-sulfate, pH 8.0 Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 chondroitin
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
4.2.2.21 chondroitin sulfate D
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 chondroitin sulfate E
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 dermatan sulfate
-
 Proteus vulgaris ?
-
 ?
4.2.2.21 hyaluronan
-
 Proteus vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.21 ? x * 110000, SDS-PAGE, x * 112614, calculated Proteus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.21 30
-
 and up to 37, substrate dermatan sulfate Proteus vulgaris
4.2.2.21 37
-
 substrate chondroitin 6-sulfate Proteus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.21 45
-
 dramatic loss of activity Proteus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.21 2.7
-
 dermatan sulfate mutant R500A, pH 8.0 Proteus vulgaris
4.2.2.21 7
-
 chondroitin 6-sulfate mutant R500A, pH 8.0 Proteus vulgaris
4.2.2.21 10
-
 dermatan sulfate mutant H712A, pH 8.0 Proteus vulgaris
4.2.2.21 19
-
 chondroitin 6-sulfate mutant H712A, pH 8.0 Proteus vulgaris
4.2.2.21 66
-
 dermatan sulfate mutant H561A, pH 8.0 Proteus vulgaris
4.2.2.21 450
-
 dermatan sulfate wild-type, pH 8.0 Proteus vulgaris
4.2.2.21 617
-
 chondroitin 6-sulfate wild-type, pH 8.0 Proteus vulgaris
4.2.2.21 652
-
 chondroitin 6-sulfate mutant H561A, pH 8.0 Proteus vulgaris
4.2.2.21 867
-
 chondroitin 4-sulfate wild-type, pH 8.0 Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.21 8
-
-
 Proteus vulgaris
4.2.2.21 9
-
 inactive Proteus vulgaris